IFF

    A simple and fast kinetic assay for phytases using phytic acid–protein complex as substrate

    The most commonly used method to assay phytase, developed nearly a century ago, measures the Pi liberated from IP6. This traditional endpoint assay is time-consuming and well known for its cumbersomeness in addition to requiring extra caution for handling the toxic regents used. This article reports a simple, fast, and nontoxic kinetic method adaptable for high throughput for assaying phytase using IP6–lysozyme as a substrate.
    Document information
    Product / service: Enzymes, Phytases
    Publication date: 02/11/2010
    Species: Poultry, Pig, Ruminants, Aquaculture
    Authors: Dalsgaard, S, Yu S, Hatti-Kaul, R, Tran, TT
    Doctype: Publications & Citations
    Publication / conference: Analytical Biochemistry
    Regions and countries: Global
    Keywords: endogenous enzyme, phosphorous tax, broiler multi-enzyme, broiler bodyweight gain, Kinetic assay for phytases, Microbial phytases, phytate poultry diets, piglet bodyweight gain, pig enzymes, poultry amylase, poultry b-glucanase, poultry carcass yield, poultry endogenous enzymes, poultry gut health, poultry phosphorus, Poultry phytase, poultry probiotics, poultry protease, poultry wheat diet, poultry xylanase, Rajni Hatti-Kaul, Shukun Yu, swine phytase, swine amylase, swine betaine, swine enzymes, swine FCR, swine profitability, swine protease, swine xylanase, Søren Dalsgaard, Thuy Thi Tran, Phytate–protein interaction
    Production challenge(s): animal producer profitability, Performance growth
    Diets: All diets
    Brands: Axtra® PHY, FASTKit assay, Optimize Feed, Phyzyme® XP
    Resource ref:
    Recently viewed

    Your single source for articles, publications & citations, product information and more...

    Much of the content on this site is freely available. Register now to receive email notifications based on your content preferences. Partners may also request access to the restricted area of the Knowledge Centre for technical product information such as trial data, reports and much more.

    Register for content notifications
    x

    DuPont's Nutrition & Biosciences and IFF are coming together

    This isn't about forming another industry giant. This is about merging curiosity and capability to create unique, leading-edge solutions at the intersection of science and creativity.

    To learn more about IFF and the merger, go to www.iff.com.