A simple and fast kinetic assay for phytases using phytic acid–protein complex as substrate

    The most commonly used method to assay phytase, developed nearly a century ago, measures the Pi liberated from IP6. This traditional endpoint assay is time-consuming and well known for its cumbersomeness in addition to requiring extra caution for handling the toxic regents used. This article reports a simple, fast, and nontoxic kinetic method adaptable for high throughput for assaying phytase using IP6–lysozyme as a substrate.
    Document information
    Product / service: Enzymes, Phytases
    Publication date: 02/11/2010
    Species: Poultry, Pig, Ruminants, Aquaculture
    Authors: Dalsgaard, S, Yu S, Hatti-Kaul, R, Tran, TT
    Doctype: Publications & Citations
    Publication / conference: Analytical Biochemistry
    Regions and countries: Global
    Keywords: endogenous enzyme, phosphorous tax, broiler multi-enzyme, broiler bodyweight gain, Kinetic assay for phytases, Microbial phytases, phytate poultry diets, piglet bodyweight gain, pig enzymes, poultry amylase, poultry b-glucanase, poultry carcass yield, poultry endogenous enzymes, poultry gut health, poultry phosphorus, Poultry phytase, poultry probiotics, poultry protease, poultry wheat diet, poultry xylanase, Rajni Hatti-Kaul, Shukun Yu, swine phytase, swine amylase, swine betaine, swine enzymes, swine FCR, swine profitability, swine protease, swine xylanase, Søren Dalsgaard, Thuy Thi Tran, Phytate–protein interaction
    Production challenge(s): animal producer profitability, Performance growth
    Diets: All diets
    Brands: Axtra® PHY, FASTKit assay, Optimize Feed, Phyzyme® XP
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